Antibacterial, Antifungal and Antihelminthic Properties of Ethanolic, Methanolic and Water Extracts of Pollen

Microorganisms and helminthes can cause serious diseases in humans as well animals. The use of antimicrobial antihelminthic drugs have created selective pressure caused resistance to antibiotics used against them, thus it necessitates the honey bee’s derived natural products. One such bee product is pollen, collected by worker bees from flowering plants modify adding its salivary secretions. present study embodies pollen substance. Among microorganisms 4 Gram (+ve) bacteria; (Bacillus subtilis, Staphylococcus epidermidis, aureus, Streptococcus pneumoniae) 3 (-ve) (Escherichia Coli, Pseudomonas aeruginosa, Salmonella enteric) 2 yeasts (Candida albicans Saccharomyces cerevisiae) were methodology disc diffusion assay broth dilution method. effect was observed among amphistomes via bioassay method under vitro conditions. For observations three types extracts (ethanolic, methanolic water extract) prepared positive controls were; Ampicillin for antibacterial, Amphotericin B antifungal Albendazole anti-helminthes. activities determined measuring zones inhibition diameters millimeters after 24 hours incubation at optimum temperature each microbe also Results obtained showed that extract found be most effective bacteria where; more susceptible compared bacteria. It yeasts; cerevisiae towards ethanolic while Candida inhibitions pollen. demonstrated none Helminthes (amphistomes) study.